Genome regulation in context: expanding understanding of genome regulatory programs by integrative analysis of signalling pathways and cellular responses controlled by the plant hormone jasmonate
- AgriBio, La Trobe University, Melbourne, VIC, Australia.
- Plant Biology Laboratory, Salk Institute, La Jolla, CA, USA.
- Howard Hughes Medical Institute, Salk Institute, La Jolla, CA, USA.
We are investigating the genome regulatory network that controls responses to the hormone jasmonate in seedlings. There is much interest currently in understanding the systems-level actions of transcription factors. However, such studies can provide much greater biological insight if set in the context of the signal transduction pathway that initiates gene expression changes and the downstream organismal phenotypes they control. We are generating an integrated framework of the response to jasmonate that spans from signal perception and transduction, through activity of master and secondary-regulatory transcription factors, to gene expression outputs and chromatin remodelling. We make use of genome-wide target maps for hundreds of transcription factors, dynamic histone modification analysis, massively multiplexed protein-protein interactome data and time series transcriptome analysis. This enables us to predict previously unknown components of the jasmonate regulatory mechanism and validate them through targeted mutant studies. The result is a comprehensive understanding of how a plant hormone remodels cellular function and plant behaviour.