A search for predictive biomarkers of ovine pre-partum vaginal prolapse

Brown SC and Norris GE

Institute of Fundamental Science, Massey University, Palmerston North, New Zealand.

Ovine pre-partum vaginal prolapse (known as bearings in sheep) occurs within a few weeks prior to lambing. Rates of prolapse vary from 0.1 to 5% per annum, varying between season and farms. Much research has been undertaken over many years to determine the cause of this condition but no clear etiology has emerged. In this study plasma samples were collected prior to prolapse occurring and were subjected to 2D DIGE (differential in gel electrophoresis) to determine if there were differences between the protein profile of ewes that were about to prolapse and control ewes. An improved method for running 2D gels was developed resulting in improved resolution and sensitivity. Results will be presented and discussed.