Impact of signalling activity on the allocation of germ layer progenitors: insights from the spatial transcriptome of gastrula-stage embryo
- Embryology Unit, Children’s Medical Research Institute.
- School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney.
Gastrulation is a critical milestone of embryogenesis at which the primary germ layers are formed and the multipotent embryonic cells are allocated to the progenitors of tissue lineages within the germ layers. Analysis of the spatial transcriptome of the epiblast showed that spatially delimited agonistic and antagonistic signalling activity in the epiblast and signalling conditions for the derivation and maintenance of the epiblast stem cells underpin the restriction of the tissue potency of epiblast cells, culminating in the generation of lineage-restricted progenitors for the germ layers and the regionalization of tissue precursors of major body parts in the germ layers during gastrulation.