Protecting the genetic code

Richard DJ1, Adams M1, Cubeddu L2, Gamsjaeger R2, Bolderson E1, Leong V1, Burgess J1, Pacquet N1 and O'Byrne K1

  1. Queensland University of Technology.
  2. Western Sydney University.

Single-stranded DNA binding (SSB) proteins play a critical role in DNA replication and repair in all three domains of life. SSB proteins all employ an oligonucleotide/oligosacharide binding (OB) fold in order to bind the single-stranded DNA substrate. Human SSB1 (hSSB1, NABP2), has been demonstrated to play key roles in the DNA damage response, especially in the process of double-stranded DNA breaks (DSBs) repair, at stalled DNA replication forks and in response to oxidative damage to DNA. In this presentation, we look at the role hSSB1 plays in the cellular response to oxidative DNA damage. Further, we will present data outlining the molecular mechanism through which hSSB1 recognises the genetic lesion and recruits the correct repair proteins for the processing of the damage. Lastly, we will present data illustrating the critical role hSSB1 plays in cancer cell biology and preliminary data on the first in class inhibitor of hSSB1. In summary, our presentation will highlight the critical role hSSB1 plays in preventing the loss of genetic information within the human genome, a process that drives cancer initiation and progression.