The role of the prorenin receptor in male fertility

Ahmady S1, Bernard P2, Merriner DJ3, Chan A4, Bagheri-Fam S1, Hobbs RM4, O'Bryan MK3, Pask AJ2 and Wilhelm D1

  1. Department of Anatomy & Neuroscience, University of Melbourne, Australia.
  2. School of BioSciences, University of Melbourne, Australia.
  3. School of Biological Sciences, Monash University, Australia.
  4. ARMI and the Department of Anatomy & Developmental Biology, Monash University, Australia.

The prorenin receptor (PRR) is best known for its tole in the renin-angiotensin-system (RAS) to regulate blood pressure and salt homeostasis. However, more recently, PRR has been shown to be a multi-functional protein, which is involved in a number of downstream pathways including MAPK signalling, protein sorting and folding and receptor-mediated endocytosis and recycling through its interaction with the vacuolar H+-ATPase (V-ATPase), as well as canonical and non-canonical WNT signalling. Given that MAPK and WNT signalling is important for ovary and testis differentiation, we hypothesised that PRR plays a role in gonadal development and function. To test this hypothesis, we deleted Prr specifically in gonadal somatic cells using the Nr5a1-Cre mouse. While these mice appear to develop normally and are born at the expected Mendelian ratio, both males and females are infertile. Here, we present our analysis to date of the testicular phenotype of the conditional Prr-null mice.