Structure of human telomerase

Rhodes D

NTU Institute of Structural Biology, Nanyang Technological University, Experimental Medicine Building, 6-03, 59 Nanyang Drive, Singapore 636921.

Telomeres, the protein-DNA complexes that cap the ends of eukaryotic chromosomes, are essential for genome stability and cell viability. In eukaryotes, telomere length is maintained by telomerase, a specialized reverse transcriptase capable of de novo DNA synthesis. Telomere length maintenance in stem cells and the majority of cancers is carried out by the specialized reverse transcriptase, telomerase. I will describe our recent 3D-structural analysis of human telomerase using single-particle cryo-EM as well as the biochemical and structural characterization of the telomerase recruitment complex.